Q&A for How to Measure the Dissolved Oxygen Level of Water

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How do I determine if there is a low or high level of dissolved oxygen?

Bess Ruff, MA
Environmental Scientist
Bess Ruff is a Geography PhD student at Florida State University. She received her MA in Environmental Science and Management from the University of California, Santa Barbara in 2016. She has conducted survey work for marine spatial planning projects in the Caribbean and provided research support as a graduate fellow for the Sustainable Fisheries Group.

Bess Ruff, MA

Environmental Scientist

Expert Answer

Each of the methods above provides a means of determining the level of dissolved oxygen in water. The first two will provide a numerical calculation of the level from which you can discern exactly whether it's high or low. The colorimetric approach will give you a general sense of the level of dissolved oxygen depending on the color produced by the reagent.

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Under the same circumstances of aeration, will water and a water-based medium (like a microbiology culture medium) have same amount of dissolved oxygen?

Daniel

Community Answer

No, water and a water-based medium like a microbiology culture medium will not necessarily have the same amount of dissolved oxygen under the same aeration circumstances. The dissolved oxygen concentration can vary based on factors such as the composition of the medium, temperature, pressure, and the presence of microorganisms. Culture media often contain additional compounds and nutrients that can affect dissolved oxygen levels differently compared to pure water.

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To obtain the rough estimation of dissolved oxygen, can we directly pour these two chemicals and observe the color change, or do we need to prepare the sample of water before carrying the same?

Daniel

Community Answer

To estimate dissolved oxygen, collect a water sample. Prepare it, removing interfering substances. Use a dissolved oxygen test kit or probe. Follow instructions for measurement. Note that this provides a rough estimate, not precise data.

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Where can I find the key for the concentration or preparation of indigo carmine solution?

Daniel

Community Answer

To find the key for the concentration or preparation of indigo carmine solution, check the manufacturer's instructions, consult chemical reference books, refer to laboratory protocols, or ask a knowledgeable colleague or supervisor.

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