How to Test for Starch

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Starch tests are used to detect the presence of starch in leaves, foods, and liquids. The process is easy and can quickly tell you if a leaf has undergone photosynthesis by its starch levels or if a food or liquid contains starch. Using iodine to test for starch is a simple way to engage your students in a classroom experiment or create an educational afternoon at home.

Method 1
Method 1 of 3:

Testing Leaves for Starch

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  1. Since this test will determine if photosynthesis has occurred in your leaf, make sure that the leaf you choose has had at least a few hours of recent exposure to sunlight. [1]
    • Although you can use any green leaf for this experiment, hibiscus leaves tend to produce better results. [2]
  2. Fill a beaker halfway with water, and allow it to come to a boil using a Bunsen burner or hot plate. Always use caution when working with heat by wearing gloves that protect against burns and other heat-related injuries. Make sure to keep your water at its boiling point. [3]
    • A Bunsen burner is a type of gas burner that heats liquids and solids in chemical experiments.
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  3. Watermark wikiHow to Test for Starch
    When placing your leaf into the boiling water, be sure to use your forceps (pincers, tongs or similar objects) to avoid any possible injury during this process. Boiling your leaf in the water will prevent any other chemical reactions from taking place in your leaf before you test for starch. This will allow you to get the most accurate results possible. [4]
    • Your leaf will be done boiling when it has become completely soft. [5]
    • Boiling your leaf will remove the waxy covering or cuticle that may prevent the entry of the iodine. [6]
  4. Watermark wikiHow to Test for Starch
    Make sure to always use forceps or tweezers to remove your leaf from the boiling water. As you remove your leaf, make sure to turn off your Bunsen burner right away. [7]
    • It is not necessary to turn off hot plates or electric water baths at this time. [8]
  5. Try to get your leaf as close to the bottom of the boiling tube as possible. Keep your ethanol away from any open flames. Always wear safety goggles when using ethanol and other chemicals. Make sure that the amount of ethanol in the boiling tube is enough to completely cover your leaf. [9]
    • A boiling tube is a cylinder tube of thin glass. It will be open on one end and will be able to withstand extreme temperatures. A test tube is suitable for this experiment.
  6. Watermark wikiHow to Test for Starch
    While your leaf is in the boiling tube, place the tube in the hot water. You should let it boil for approximately 10 minutes, however, if your leaf decolorizes before that, go ahead and remove it from the heat. [10]
    • It is better to use a hot plate or water bath for this step as ethanol is extremely flammable.
    • You may need to replenish your ethanol levels if the ethanol is no longer covering your leaf completely. [11]
    • Your leaf is done boiling when all of the green coloring is transferred to the ethanol, leaving your leaf colorless. [12]
  7. Watermark wikiHow to Test for Starch
    Use your forceps to gently remove your entire leaf from the boiling tube. Ensure that your leaf stays intact during the removal process and rinse with a tiny bit of cold water. Make sure you are wearing your protective goggles and heat-resistant gloves. [13]
    • Boiling your leaf in ethanol will cause it to become brittle. By adding a small amount of cold water, it will help the leaf regain a soft texture.
  8. Watermark wikiHow to Test for Starch
    Make sure that as you place your leaf on the tile that you are evenly distributing the area of your leaf. This will help provide clarity when looking at the results of your starch test. [14]
    • You can also place your leaf in a petri dish, rather than placing it directly on the white tile. [15]
  9. Watermark wikiHow to Test for Starch
    While a few drops should be enough to cover your entire leaf, continue to add 1 drop at a time if your leaf is not fully covered. [16]
    • Soaking your leaf is not necessary, but it will increase the accuracy of your results. [17]
  10. If starch is present in your leaf, the iodine solution will have changed color from brown to blue-black. If there was no change in your iodine solution and the liquid remains brown, there is no starch present in your leaf. [18]
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Method 2
Method 2 of 3:

Searching for Starch in Foods

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  1. You can test for starch in virtually any type of food. Many prefer to work with fruits and vegetables but you can also test for starch in breads and sugars. Also, grab a non-starchy food to act as your control. Use foods such as apples and cucumbers for your control samples. Make sure that your slices are thin. [19]
    • Lighter colored foods will work best for this experiment. [20]
  2. Watermark wikiHow to Test for Starch
    Small, disposable cups will help you keep your samples from crossing with other samples that could potentially skew your results. It will also help contain the iodine solution when you apply it to your samples. [21]
  3. Watermark wikiHow to Test for Starch
    Making one of your samples your control means you will not be adding the iodine to it. This sample will be used to determine if your other sample has changed in the iodine. [22]
    • Control samples are not required but can be very useful when analyzing your results.
  4. Watermark wikiHow to Test for Starch
    Make sure to keep your control sample separate from the sample you are applying the iodine too. Do not use the sample you added water to. [23]
    • You can add the iodine directly to your samples or place the iodine in the bottom of a cup, lay your sample on a paper towel, and place it on top of the iodine. [24]
    • If you are unable to find an iodine solution you can also use Betadine (povidone-iodine mix), Lugol’s (a mixture of iodine and potassium), or a tincture (where the iodine is dissolved in water or alcohol). [25]
  5. If your sample has turned blue-black in color, then starch is present. When comparing your sample to the control, your control samples, even after adding the water, will have kept their original color. Your iodine will have turned blue-black if starch is present in your food. If your sample looks brown, then there is no starch present. [26]
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Method 3
Method 3 of 3:

Conducting a Liquid Starch Test

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  1. The iodine starch test can be run on almost any liquid. However, you should avoid using dark liquids as they will not allow you to observe the appropriate color change when determining your results. [27]
  2. Watermark wikiHow to Test for Starch
    Adding your liquids to test tubes will make it easier keep the sample you are adding iodine to separate from your controls. When using more than one sample, this will also help you avoid cross-contamination. [28]
  3. Watermark wikiHow to Test for Starch
    You will need to fill your test tube at least a quarter of the way full, but do not exceed the halfway point when adding your sample.
  4. Watermark wikiHow to Test for Starch
    The number of drops you add will depend on the amount of liquid you placed in your test tube. [29] For smaller samples add 1-2 drops of the iodine solution. Your iodine solution should be added directly to your liquid in the test tube. There is no need to stir the mixture, just allow the iodine to settle into your liquid.
    • If you opted to place your liquid on a tile, add the drop of iodine directly to the liquid.
  5. Often there will be a black tint to the blue, which still indicates that your sample contains starch. If the color of the iodine has remained brown, starch is not present in your liquid. [30]
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Expert Q&A

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  • Question
    Does cold pasta have resistant starch?
    Claudia Carberry, RD, MS
    Registered Dietitian
    Claudia Carberry is a Registered Dietitian based in Little Rock, Arkansas. Claudia specializes in kidney transplants and counseling patients for weight loss. She works at the University of Arkansas for Medical Sciences center, and has been working there since 2015. Before her current role, she worked as a Clinical Nutrition Manager at Jefferson Regional Medical Center. She is a member of the Arkansas Academy of Nutrition and Dietetics. Claudia received her MS in Nutrition from the University of Tennessee, Knoxville, where she completed her 1200 hour dietetic internship. She received a BS in Nutrition Sciences from Southeast Missouri State University.
    Claudia Carberry, RD, MS
    Registered Dietitian
    Expert Answer
    No, pasta is not a good source of resistant starch. Try eating corn and potatoes instead.
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  • Question
    What is the control for starch testing?
    Athena21
    Community Answer
    The control would be doing this procedure without the biotic element. For example, the control for testing for starch in a potato would be just putting iodine solution on a tile without the potato.
    Thanks! We're glad this was helpful.
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  • Question
    How do you differentiate blue, black and brown for this type of test?
    Ethansciencelab
    Community Answer
    Blue or black that means there is starch found, but brown means there is no starch.
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    Thank you for your feedback.
    If wikiHow has helped you, please consider a small contribution to support us in helping more readers like you. We’re committed to providing the world with free how-to resources, and even $1 helps us in our mission. Support wikiHow
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      Warnings

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      Things You'll Need

      Testing Leaves for Starch

      • Bunsen burner or hot plate
      • 250 mL (8.5  fl oz) beaker
      • Boiling tube
      • Forceps
      • Test tube rack
      • White tile
      • A leaf to be tested
      • 90% ethanol
      • Iodine Solution
      • Eye protection goggles
      • Heat resistant gloves

      Searching for Starch in Foods

      • Dropping pipette
      • Iodine Solution
      • A starchy food such as a raw potato, pasta, or bread
      • A non-starchy food such as apples, cucumbers, or pure sugar.
      • Small, disposable, plastic cups
      • Newspaper to cover your work area

      Conducting a Liquid Starch Test

      • A light-colored liquid
      • Test tube
      • Test tube rack
      • Glass Rod to stir
      • Dropping pipette
      • Eye protection
      • Iodine Solution

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      References

      1. ↑ https://practicalbiology.org/standard-techniques/testing-leaves-for-starch-the-technique
      2. ↑ https://www.youtube.com/watch?v=E9CSyXS3pXc
      3. ↑ https://practicalbiology.org/standard-techniques/testing-leaves-for-starch-the-technique
      4. ↑ http://www.preproom.org/practicals/pr.aspx?prID=1037
      5. ↑ https://www.youtube.com/watch?v=E9CSyXS3pXc
      6. ↑ https://practicalbiology.org/standard-techniques/testing-leaves-for-starch-the-technique
      7. ↑ https://www.youtube.com/watch?v=E9CSyXS3pXc
      8. ↑ https://practicalbiology.org/standard-techniques/testing-leaves-for-starch-the-technique
      9. ↑ https://www.youtube.com/watch?v=E9CSyXS3pXc
      1. ↑ http://brilliantbiologystudent.weebly.com/testing-a-leaf-for-the-presence-of-starch.html
      2. ↑ http://brilliantbiologystudent.weebly.com/testing-a-leaf-for-the-presence-of-starch.html
      3. ↑ http://brilliantbiologystudent.weebly.com/testing-a-leaf-for-the-presence-of-starch.html
      4. ↑ http://brilliantbiologystudent.weebly.com/testing-a-leaf-for-the-presence-of-starch.html
      5. ↑ http://brilliantbiologystudent.weebly.com/testing-a-leaf-for-the-presence-of-starch.html
      6. ↑ http://www.nuffieldfoundation.org/practical-biology/testing-leaves-starch-technique
      7. ↑ http://www.nuffieldfoundation.org/practical-biology/testing-leaves-starch-technique
      8. ↑ http://www.nuffieldfoundation.org/practical-biology/testing-leaves-starch-technique
      9. ↑ http://brilliantbiologystudent.weebly.com/testing-a-leaf-for-the-presence-of-starch.html
      10. ↑ http://kitchenpantryscientist.com/starch-test/
      11. ↑ http://kitchenpantryscientist.com/starch-test/
      12. ↑ http://www.webexhibits.org/causesofcolor/6AC.html
      13. ↑ http://kitchenpantryscientist.com/starch-test/
      14. ↑ http://www.webexhibits.org/causesofcolor/6AC.html
      15. ↑ http://www.webexhibits.org/causesofcolor/6AC.html
      16. ↑ http://www.webexhibits.org/causesofcolor/6AC.html
      17. ↑ http://kitchenpantryscientist.com/starch-test/
      18. ↑ http://brilliantbiologystudent.weebly.com/iodine-test-for-starch.html
      19. ↑ http://brilliantbiologystudent.weebly.com/iodine-test-for-starch.html
      20. ↑ http://brilliantbiologystudent.weebly.com/iodine-test-for-starch.html
      21. ↑ http://brilliantbiologystudent.weebly.com/iodine-test-for-starch.html
      22. ↑ http://www.preproom.org/practicals/pr.aspx?prID=1037
      23. ↑ http://www.preproom.org/practicals/pr.aspx?prID=1037

      About This Article

      Article Summary X

      To test foods for starch, start by cutting your food into small slices and placing them in disposable plastic cups to keep your samples clean. Once your test foods are ready, add 1 drop of water to the cup that contains your control sample. After you control sample is ready, add 1 to 2 drops of iodine to the sample being tested, putting the iodine directly onto your sample or placing it in the bottom of the cup. Notice if your iodine has turned blue-black in color, which indicates that starch is present. If, on the other hand, your sample looks brown, there’s no starch present. For more tips from our Science co-author, including how to test for starch in liquids, keep reading!

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